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The Leica SP8 is an automated inverse confocal laser scanning microscope allowing simultaneous aquisition of 3 fluorescent channels and 1 transmitted light channel (BF or DIC).
University of Zurich, Forschungszentrum für das Kind FZK, August-Forel-Strasse 51, 8008 Zürich, room: LLF.C+4.020
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Dual scanning system:
Regular scanner: 10 - 1800 Hz
Resonant scanner: 8000 Hz
| Name | Magnification | NA | Immersion | WD (mm) |
|---|---|---|---|---|
| HC PL APO | 10x | 0.3 | Air | 11 |
| HC PL APO CS2 | 20x | 0.75 | Imm | 0.67 |
| HCX PL APO 37°C CS2 | 63x | 1.3 | Glycerol | 0.3 |
Two Hybrid (HyD) detectors, one photomultiplier tube (PMT) detector and one additional PMT for transmission. Excitation controlled by AOTF, beam splitters: RT 15/85, substrate, TD 488/552/638, DD 488/552 for separation between excitation and emission. Accurate emission detection by spectral detectors.
| Name | Excitation Range | Excitation Filter | Dichroic | Emission Filter |
|---|---|---|---|---|
| A4 | UV | BP 360/40 | 400 | BP 470/40 |
| GFP | blue | BP 470/40 | 500 | BP 525/50 |
| N2.1 | green | BP 515-560 | 580 | LP 590 |
| CFP | violet/blue | BP 436/20 | 455 | BP 480/40 |
| I3 | blue | BP 450/490 | 410 | LP 515 |
This microscope acquires images with improved axial resolution compared with more coventional widefield microscopes. In addition to the multicolor images the microscope can acquire large tile scans fully automatically. The microscope allows live imaging with controlled environmental conditions (CO2, temperature, humidity).
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