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The Leica STELLARIS 5 is an automated inverse confocal laser scanning microscope allowing simultaneous acquisition of 3 fluorescent channels as well as a transmission bright field channel. The newest TauSense technology gives instant access to lifetime-based information, delivering additional insights to your experiments.This system is ideal for fixed samples.
University Zurich Center, at the USZ Schmelzbergstrasse 12, Room PATH A25.
Follow this link to apply for an introduction to the microscope.
Scanners
Regular scanner: 1 - 2600 Hz
Objectives
| Name | Magnification | NA | Immersion | WD (mm) |
|---|---|---|---|---|
| HC PL FLUOTAR | 5 | 0.15 | Air | 13.70 |
| HC PL APO CS2 | 10 | 0.4 | Air | 2.56 |
| HC PL APO CS2 | 20x | 0.75 | Air | 0.62 |
| HC PL APO CS2 | 20x | 0.70 | IMM | 0.26-0.17 |
| HC PL APO CS2 | 63x | 1.4 | Oil | 0.14 |
CS2 objectives: improved color correction, perfect VIS-405; IMM = multi immersion (either water, glycerol or oil)
3 Hybrid (Power HyD S) detectors, and one additional detector for transmission (Trans PMT). Excitation controlled by AOTF. Accurate emission detection by spectral detectors.
| Name | Excitation Range | Excitation Filter | Dichroic | Emission Filter |
|---|---|---|---|---|
| DAPI. | UV | BP 405/60 | 455 | BP 470/40 |
| GFP | blue | BP 470/40 | 495 | BP 525/50 |
| RHOD | green | BP 546/10 | 560 | BP 585/40 |
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy
This microscope acquires images with improved axial resolution compared with more coventional widefield microscopes.The newest TauSense technology gives instant access to lifetime-based information, delivering additional insights to your experiments. In addition to the multicolor images in fixed samples the microscope can acquire large tile scans fully automated thanks to the LAS X Navigator.
Follow this link for further background information, documents and links
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy
