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The ImageXpress Confocal HT.ai High-Content imaging system utilizes a seven-channel laser light source (410 nm - 750 nm) with eight imaging channels to enable highly multiplexed assays. Designed for experiments involving live cells as the spinning disk reduces phototoxicity and enables fast acquisition. The system supports slides and one to 1536-well microplates, round or flat bottom, low to high profile.
University Zurich, Irchel Campus, Room Y42-H-91.
Follow this link to apply for an introduction to the microscope.
Microscope
Dual disk unit allowing selection between 3 modes:
50 micron (named 51 micron slit) for high throughput confocal requirements (faster acquisition but lower optical sectioning). Spacing between pinholes 250 um
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Default installed objectives |
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|---|---|---|---|---|---|---|
| Name | Mag | NA | Immersion | WD (mm) | Pixel size (um/pixel) |
Recommended z step for deconvolution (um) |
| Nikon LWD Lambda S 40XC WI | 40x | 1.15 | Water | 0.61–0.59 |
0.1687 |
0.2 |
| Nikon CFI Apo LWD Lambda S 20XC WI | 20x | 0.95 | Water | 0.99–0.9 | 0.344 | 0.3 |
| Nikon CFI Plan Apo Lambda 10X | 10x | 0.45 | Air | 4 | 0.678 | |
| Nikon CFI Plan Apo Lambda 40XC | 40x | 0.95 | Air | 0.25–0.17 | ||
| Optional objectives to be installed on request | ||||||
| Nikon CFI Plan Apo Lambda 20X | 20x | 0.75 | Air | 1 |
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| Nikon CFI Plan Apo Lambda 2X | 2x | 0.10 | Air | 8.5 | ||
| Channel | Excitation Laser (nm) | Common Fluorophores | Emission Filter |
|---|---|---|---|
| DAPI | 405/20 | DAPI, Hoechst, Alexa Fluor 350, BFP | 452/45 nm |
| CFP | 445/20 | CFP | 483/32 nm |
| FITC | 470/21 | FITC, GFP, FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green | 520/28 nm |
| YFP | 520/10 | YFP | 562/40 nm |
| TRITC | 555 | Cy3, TRITC, Alexa Fluor 532 & 546 & 555, Rhodamine, TAMRA, LysoTracker Yellow, MitoTracker Orange |
598/25 nm |
| Texas Red | 555 | Texas Red, mCherry, Alexa Fluor 568 & 594, Calcium Crimson, Cy3.5, HcRed, MitoTracker Red, Propidium Iodide | 624/40 nm |
| Cy5 | 638/17 | Cy5, Alexa Fluor 647 & 660, DRAQ5, APC (Allophycocyanin), BODIPY 650/665, DiD, SYTO Red, TOTO-3, and TO-PRO-3 | 692/40 nm |
| Cy7 | 725/30 | Cy7 | 794/32 |
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy
In order to ensure reliable imaging at this instrument we recommend using imaging supports with high performance #1.5 cover glass or equivalent. The following imaging supports have been successfully used:
If you'd like to ask for a free sample to test, please use the link https://ibidi.com/content/875-ibidi-free-sample-program
We do not support or troubleshoot autofocus strategies using ultra-thin transparent film bottoms.
Please contact us in case you have further question.
