HCS - Perkin Elmer Operetta (Lengg) | Center for Microscopy and Image Analysis

From everyday assays to more demanding applications, the Operetta CLS (spinning disk) high-content analysis system delivers just the right combination of flexible excitation, sensitive optics, and advanced software features to enable you to gain deeper biological insight from all your critical applications.

Location

University of Zurich, Forschungszentrum für das Kind FZK, August-Forel-Strasse 51, 8008 Zürich, room: LLF.C+4.020

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

Precisicon autofocus unit
Dual Nipkow spinning disk for optical sections
Widefield (not-confocal) imaging mode

Objectives

Installed objectives
Name	Mag	NA	Immersion	WD (mm)	Field of View (µm²)
Opera PHX/OPRTCLS HH14000422	40x	1.1	Water	0.62	338 x 254
Opera PHX/OPRTCLS HH14000421	20x	1.0	Water	1.7	646 x 646
DACH CLS	20x	0.4	Air	8.28	646 x 646
Opera PHX/OPRTCLS HH14000403	10x	0.3	Air	5.2	1292 x 1292
Opera PHX/OPRTCLS HH14000402	5x	0.16	Air	12.1	2583 x 2583

LED Excitation Light

Excitation Laser (nm)	Common Fluorophores	Emission Filter (nm)
365	Live/Dead Fix Aqua, Marina Blue, Fura-2	360-400
405	DAPI, Hoechst, Alexa Fluor 350, BFP	460-490
440	CF440, ER-ID Green	500-550
475	FITC, GFP, FAM, Alexa Fluor 488, BODIPY, Calcein, Fluo-4, MitoTracker Green	570-650
510	YFP	570-650
550	Cy3, TRITC, Alexa Fluor 532 & 546 & 555, Rhodamine, TAMRA, LysoTracker Yellow, MitoTracker Orange	570-650
550	Texas Red, mCherry, Alexa Fluor 568 & 594, Calcium Crimson, Cy3.5, HcRed, MitoTracker Red, Propidium Iodide	655-760
630	Cy5, Alexa Fluor 647 & 660, DRAQ5, APC (Allophycocyanin), BODIPY 650/665, DiD, SYTO Red, TOTO-3, and TO-PRO-3	655-760
660	Cy7	655-760

Camera Systems

sCMOS camera with 4.7 megapixels (2160 x 2160), 16 bit resoltuion and 6.5 µm pixel size

Accessories

4 slide sample holder
Climate control Opera PHX/OPRTCLS: The sample chamber is equipped with temperature and CO2 control for live cell experiments. The temperature can be regulated between 37°C and 42°C and the CO2 concentration between 1% and 10%

Literature and Links

Perkin Elmer Operetta Resources

ibiology - Optical sectioning and confocal microscopy

Spinning Disk Confocal Microscopy

Responsible Persons

If you have questions about the device please contact the responsible person.

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

Recommended imaging supports

In order to ensure reliable imaging at this instrument we recommend using imaging supports with high performance #1.5 cover glass or equivalent. The following imaging supports have been successfully used:

96 well plate ibidi - P96-1.5H-N (#1.5 glass bottom, Cat.No:89627)
96 well plate ibidi - P96-1.5P (#1.5 glass-like polymer coverslip bottom, Cat.No:89626)

If you'd like to ask for a free sample to test, please use the link https://ibidi.com/content/875-ibidi-free-sample-program

We do not support or troubleshoot autofocus strategies using ultra-thin transparent film bottoms.

Please contact us in case you have further question.

Center for Microscopy and Image Analysis

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HCS - Perkin Elmer Operetta (Lengg)