Spinning Disk- Olympus (Irchel)

The Olympus IXplore SpinSR10 super resolution imaging system is a high-end real time spinning disk confocal imaging system. 

It is equipped with two sCMOS cameras, which allow fast and sensitive simultaneous acquisition of two-color labelled (live) samples.

Designed for experiments involving live cells, the spinning disk (YOKOGAWA CSU-W1) reduces phototoxicity and bleaching while the Olympus Z-drift compensator (IX3-ZDC2) maintains focus.

The photomanipulation unit (Rapp OptoElectronic) allows localized and controlled photostimulation as well as damage/ablation in biological samples. 

The stage top incubation cellVivo system allows control of temperature and CO₂ levels enabling live cell experiments.

University Zurich, Irchel Campus, Room Y42-H-83. 

Follow this link  to apply for an introduction to the microscope.

Microscope

Light Sources and Lasers

• Laser lines: 405 nm (50 mW), 488 (100mW), 561 nm (100 mW), 640nm (100mW), 445nm (75nm) and 514nm (40mW).
• Photomanipulation unit: 355 nm (pulsed, UGA‑42 Caliburn), 405 nm (100 mW), 473 nm (100mW)
• CoolLED’s pE-300 LED illumination for widefield fluorescence microscopy (3 LEDs). Spectral coverage from the UV (DAPI excitation) to the red region (Cy5 excitation)

Objectives

Additional objectives (not currently installed)

Fluorescence Filters

Confocal Imaging Filters (implemented in CSU-W1)

Widefield

For further details please contact the responsible persons.

Camera Systems

• 2x Hamamatsu ORCA-Fusion sCMOS
• 2304 x 2304 pixel (6.5 x 6.5um pixel size)
• up to 89.1 fps @ 16-bit (at 2304 x 2304 pixel)

Accessories

• Inserts for ibidi slides, well plates and 35mm dishes
• Incubation system for live cell imaging (cellVIVO)
• Allows for a variety of experimental protocols with 0 - 20 % CO₂.
• Reliable control of temperature and CO₂ with up to 0.05 °C accuracy

Literature and Links

Guides - Spinning disk Olympus

ibiology - Optical sectioning and confocal microscopy 

Spinning Disk Confocal Microscopy

Responsible Persons

If you have questions about the device please contact the responsible person.

Example of Materials and Methods when using this instrument (please adjust accordingly with your specific settings) 

Single plane / Maximum intensity projections (MIP) of x um stacks were acquired using Olympus IXplore SpinSR10 spinning disk confocal microscope, equipped with a Yokogawa CSU-W1 50 μm pinhole disk and a 00x  magnification x air / immersion media objective. Sequential laser excitation was performed using 405nm, 488nm, 561nm. Emission was detected through the following band pass filters Fluorophore 1  (447/60), Fluorophore 2 (525/50) Fluorophore 3  (617/73) with a Hamamatsu ORCA-Fusion sCMOS camera (2304 x 2304 pixel, 6.5 x 6.5um pixel size). 

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