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The Spinning Disk Microscope is a high-end real time confocal imaging system, equipped with the Yokogawa Confocal scanning unit W1 (2 sets of spinning disks for low and high resolution imaging).
It is further equipped with two EMCCD cameras, which allow for fast and sensitive simultaneous acquisition of two-color (live) samples, also well suited for low fluorescence emission.
The spinning disk facilitates long time investigations (hours to days) of cells and tissue, showing reduced phototoxicity and bleaching, while the Perfect Focus System (PFS, active focus control) maintains the focus.
A stage top incubation system allows control of temperature and C02 levels enabling live cell experiments.
University Zurich, Irchel Campus, Room Y24-F-14.
Follow this link to apply for an introduction to the microscope.
Microscope
| Name | Magnification | NA | Immersion | WD (mm) |
|---|---|---|---|---|
| CFI PlanApo | 10x (DIC) | 0.45 | Air | 4.0 |
| CFI S PlanFluor ELWD | 40x (Ph) | 0.6 | Air | 2.8-3.6 |
| CFI S PlanFluor ELWD | 20x (Ph) | 0.45 | Air | 6.9-8.2 |
| CFI PlanApo VC | 60x (DIC) | 1.4 | Oil | 0.13 |
| CFI PlanApo | 100x (DIC) | 1.4 | Oil | 0.13 |
| CFI SR HP PlanApo Lambda S | 100x (DIC) | 1.35 | Silicon oil | 0.28-0.31 |
| Additional Objective - not installed | ||||
| CFI PlanFluor | 20x (DIC) | 0.75 | IMM | 0.33-0.35 |
IMM = multi immersion (either water, glycerol or oil)
Mainly used if 1 camera imaging is desired. All images acquired with CAM1.
| Laser Line | Excitation Range | Dichroic (DM 1) | Emission filter (FW) | TwinCam Unit |
|---|---|---|---|---|
| 405 | UV | 410 | BP 460/50 | empty (no DM2, no EM1) |
| 488 | blue | 504 | BP 525/50 | empty (no DM2, no EM1) |
| 561 | green | 582 | BP 609/54 | empty (no DM2, no EM1) |
| 640 | red | 669 | BP 700/75 | empty (no DM2, no EM1) |
Mainly used if 2 camera imaging is desired.
| Laser Lines | FW | Image Splitter (DM 2) | EM /Camera 1 | EM /Camera 2 |
|---|---|---|---|---|
| 405/561 | open | A561 LP |
BP 605/52 (or BP 595/33) |
BP 460/50 |
| 405/640 | open | A561 LP | BP 700/75 | BP 460/50 |
| 488/561 | open | A561 LP | BP 605/52 (or BP595/33) | BP 525/50 |
| 488/640 | open |
A561 LP or A647 LP |
BP 700/75
|
BP 525/50 |
| 561/640 | open | A647 LP | BP 700/75 |
BP 595/33 BP 605/52 (only for TwinCam single) |
| LED (Excitation range) | Excitation Filter | Dichroic | Emission Filter | FW |
|---|---|---|---|---|
| DAPI (UV) | BP 390/18 | acc. QUAD | acc. QUAD | BP 460/50 |
| GFP (blue) | BP 485/20 | acc. QUAD | acc. QUAD |
BP 525/50 |
| mCherry (green) | BP 560/25 | acc. QUAD | acc. QUAD | BP 609/54 |
| Cy5 (red) | BP 650/13 | acc. QUAD | acc. QUAD | BP 700/75 |
| QUAD (UV/blue/green/red) |
BP 387/18; 485/30; 559/30; 649/22 |
410; 504; 582; 669 |
BP 440/50; 521/30; 607/40; 700/60 |
The QUAD Filter cube is only used for Epi-Fluorescence imaging. In confocal mode, filters in the microscope stand are automatically removed from the light path. For dual Cam imaging, FW needs to be open, and the appropriate EM 1/2 as well as corresponding DM 2 have to be inserted into the TwinCam.
For further imaging methods/illumination pathway options please contact the responsible person.
Follow this link for further background information, documents and links.
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy
