Widefield - Olympus ScanR HCS (Irchel)
ScanR is a modular microscope-based imaging platform designed for fully automated image acquisition and data analysis of biological samples. ScanR can handle many different formats, including multi-well plates, slides and custom-built arrays. This unmatched flexibility makes it equally adept at routine and advanced applications.
Location
University Zurich, Irchel Campus, Room Y24-F-14.
Training Request
Follow this link to apply for an introduction to the microscope.
Technical Specifications
Microscope
Motorized Olympus high-end research microscope IX83 with motorized Z-drive.
Light Sources
- High power illumination system: Lumencor SpectraX light engine (new installed Nov 2024).
Objectives
| Name | Magnification | NA | Immersion | WD (mm) |
|---|---|---|---|---|
| UPLSAPO | 4x | 0.16 | Air | 13 |
| UPLSAPO | 10x | 0.4 | Air | 3.1 |
| UPLSAPO | 20x | 0.75 | Air | 0.6 |
| UPLSAPO | 40x | 0.9 | Air | 0.18 |
| UPLFLN | 10x Phase | 0.3 | Air | 10 |
| PLAPON | 60x Phase | 1.42 | Oil | 0.15 |
Fluorescence Filters
Fluorescence filters
| Name | Exc until 2024.11 | Exc from 2024.11 | Power (mW) | Dichroic |
Emission (Fast emission filter wheel) |
|---|---|---|---|---|---|
| DAPI | BP 395/25 | BP 390/22 | 270 | 409 | BP 435/26 |
| FITC | BP 470/24 | BP 475/28 | 226 | 493 | BP 515/30 |
| Cy3 (previously TRITC) | BP 550/15 (previously TRITC) | BP 555/28 | 470 | 573 | BP 595/40 |
| Cy5 | BP 640/30 | BP 635/22 | 208 | 652 | BP 705/72 |
| Cy7 | not available | 740/20 | 117 | 759 | BP 809/81 |
| CFP | 438/29 | 405 | |||
| not installed (mCherry) | 575/25 | 351 |
Camera Systems
- Hamamatsu ORCA-FLASH 4.0 V2, high-sensitivity digital monochrome scientific cooled sCMOS camera, QE > 70% @ 600 nm, dynamic range 4000:1, 2048 x 2948 pixel of size 6.5 x 6.5 um, 12 bit dynamics, max. exposure time 10s. (recommended for low signal levels and high field-of-view applications).
Accessories
- Controlled environmental conditions (CO2, temperature) CellVivo incubation system
- Multiwell insert
- ScanR Analysis software: Automated image and data analysis for standard assays and assay development. Online and offline multicore analysis. Image processing, image analysis, particle detection, and parameter extraction and calculation. Cytometric data exploration, analysis, gating, and classification
Powerful and flexible gating concept including automated population detection
Direct link between data points, objects, and images
Assays-based work flow and advanced scientific assay development functionality
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy