Widefield - Olympus ScanR HCS (Irchel) | Center for Microscopy and Image Analysis

ScanR is a modular microscope-based imaging platform designed for fully automated image acquisition and data analysis of biological samples. ScanR can handle many different formats, including multi-well plates, slides and custom-built arrays. This unmatched flexibility makes it equally adept at routine and advanced applications.

Location

University Zurich, Irchel Campus, Room Y24-F-14.

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

Motorized Olympus high-end research microscope IX83 with motorized Z-drive.

Light Sources

High power LED illumination system: Lumencor SpectraX light engine (new installed Nov 2024).

Objectives

Name	Magnification	NA	Immersion	WD (mm)
UPLSAPO	4x	0.16	Air	13
UPLSAPO	10x	0.4	Air	3.1
UPLSAPO	20x	0.75	Air	0.6
UPLSAPO	40x	0.9	Air	0.18
UPLFLN	10x Phase	0.3	Air	10
PLAPON	60x Phase	1.42	Oil	0.15

Fluorescence Filters

Fluorescence filters

Name	Exc until 2024.11	Exc from 2024.11	Power (mW)	Dichroic	Emission (Fast emission filter wheel)
DAPI	BP 395/25	BP 390/22	270	409	BP 435/26
FITC	BP 470/24	BP 475/28	226	493	BP 515/30
Cy3 (previously TRITC)	BP 550/15 (previously TRITC)	BP 555/28	470	573	BP 595/40
Cy5	BP 640/30	BP 635/22	208	652	BP 705/72
Cy7	not available	740/20	117	759	BP 809/81
CFP		438/29	405
not installed (mCherry)		575/25	351

Camera Systems

Hamamatsu ORCA-FLASH 4.0 V2, high-sensitivity digital monochrome scientific cooled sCMOS camera, QE > 70% @ 600 nm, dynamic range 4000:1, 2048 x 2948 pixel of size 6.5 x 6.5 um, 12 bit dynamics, max. exposure time 10s. (recommended for low signal levels and high field-of-view applications).

Accessories

Controlled environmental conditions (CO2, temperature) CellVivo incubation system
Multiwell insert
ScanR Analysis software: Automated image and data analysis for standard assays and assay development. Online and offline multicore analysis. Image processing, image analysis, particle detection, and parameter extraction and calculation. Cytometric data exploration, analysis, gating, and classification
Powerful and flexible gating concept including automated population detection
Direct link between data points, objects, and images
Assays-based work flow and advanced scientific assay development functionality

Responsible Persons

If you have questions about the device please contact the responsible person.

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

Center for Microscopy and Image Analysis

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Widefield - Olympus ScanR HCS (Irchel)