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Akoya’s Vectra Polaris (newly called PhenoImager™ HT) is an automated imaging system for performing whole slide scans of tissue sections and microarrays (TMAs), and for acquiring multispectral (MSI) regions of interest. The system has been optimized to image samples stained with Akoya’s Opal multiplexed fluorescent immunohistochemistry reagent kits and is also compatible with typical brightfield staining reagents. The Vectra Polaris is configured to store and inventory up to 80 slides.
University Zurich, Schlieren Campus, Wagistrasse 12, WAD-K-105 (5th floor)
Follow this link if you want to apply for an introduction to the microscope.
Microscope
Available options for fluorescent imaging
|
Pixel Size (Scan Setting) |
Objective | Comment |
| used only for preview | 4x / 0.2 NA | |
| 1.0 µm 10x | 10x / 0.45 NA | 2x2 binning applied |
| 0.5 µm 20x (fast acquisition) | 10x / 0.45 NA | |
| 0.5 µm 20x (better quality) | 20x / 0.75 NA | 2x2 binning applied |
| 0.25 µm 40x | 20x / 0.75 NA |
Brightfield images are always recorded with 20x and final resolution is achieved by binning. The tube lens doubles the objective magnification if no binning is applied.
| Filter | Band | Excitation (nm) | Emission (nm) | Alternative Dyes |
|---|---|---|---|---|
| Opal dyes | ||||
| DAPI+Opal 570/690 | DAPI | 391/32 | 453/30 | DAPI, Hochst |
| Opal 570 | 539/25 | 571/20 | Alexa 555, RFP, tdTomato | |
| Opal 690 | 649/50 | 709/40 | Alexa 647, Cy5 | |
| Opal 520 | Opal 520 | 486/30 | 532/18 | FITC, Alexa 488, GFP |
| Opal 480/620/780 | Opal 480 | 424/42 | 478/40 | |
| Opal 620 | 591/25 | 633/40 | Alexa 594, Alexa 568 | |
| Opal 780 | 735/40 | 833/135 | ||
| Additional filters primarily used for multispectral imaging (MSI) | ||||
| DAPI+Opal 780 MSI | DAPI | 387/12 | 555/291 | |
| Opal 780 | 735/40 | 808/85 | ||
| FITC | FITC | 480/40 | 510/LP | |
| Cy3 | Cy3 | 545/25 | 570/LP | |
| Texas Red | Texas Red | 578/22 | 657/106 | |
| Opal 480 + Cy5 MSI | Opal 480 | 424/42 | 518/125 | |
| Cy5 | 620/60 | 700/76 | ||
Note: DAPI, Opal 480, Opal 520, Opal 570, Opal 620, Opal 690 and Opal 780 can be combined and recorded without multispectral imaging (significantly faster acquisition). Adding Opal 540 or Opal 650 to the panel will require multispectral recordings.
To help you get familiar with the slidescanner and its software, please read the following operating manual:
Polaris User Manual (PDF, 3 MB)
Manuals for corresponding software Phenochart and inForm:
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy
