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Center for Microscopy and Image Analysis

Slidescanner - Zeiss Axio Scan.Z1 (Schlieren)

Slidescanner

The Axio Scan.Z1 is an advanced slide scanner designed for the automated imaging of large volumes of slides, including both brightfield and multi-channel fluorescence specimens. Notably, the Axio Scan.Z1 is equipped with high numerical aperture dry objectives, ensuring exceptional resolution, and it possesses the capability to scan entire 3D volumes.

Location

University Zurich, Campus Schlieren , WAD H127 (325)

Wagistrasse 12, 8952 Schlieren

 

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

  •  Automated upright slide scanning microscope
  •  Slide holders for standard slides (26 x 76mm) or big slides (52 x 76mm) upon request

Light Sources

  • Transmitted light LED
  • HXP fluorescence lamp

Objectives

Name Magnification NA Immersion WD
Fluar 2.5x 0.12 air  
Fluar 5x 0.25 air 12.5 mm
Plan Apochromat 10x 0.45 air 2.1 mm
Plan Apochromat 20x 0.8 air 0.55 mm
Plan Apochromat 40x 0.95 air 0.25 mm

Fluorescence Filters

Name  Excitation  Beam splitter Emission
Dapi  385 FT 420 BP 450/40
CFP 436/20 FT 455 BP 480/40
eGFP 470 FT 495 BP 525/50
YFP 500/25 FT515 BP 535/30
Cy3 555 FT 570 BP 605/70
mRFP 590 FT 605 BP 647/70
Cy5 630 FT 660 BP 690/50

Camera Systems

  • Hitachi HV-F202FCL camera 8bit (color camera), 1600 x 1200 pixels (2 MP), pixel size 4.4 µm

  •  Hamamatsu Orca Flash 4.0 monochrome camera 16bit (fluorescence camera), 2048 x 2048 pixels (4 MP), pixel size 6.5 µm

Accessories

  • ZEN blue (installed on all VMs)
  • ZEN Image Analysis module (installed on Special VM1)
  • CZI to .TIFF converter (installed on all VMs)
  • ZEN Advanced Processing and Analysis module and macro environment (Python) for the setup of feedback experiments

Remarks

Literature and Links

For more in-depth information about ZEN blue:

ZEN 2 slidescan module guide (PDF, 13 MB)

Useful tips and tricks to optimize your scan profiles:

Axio Scan.Z1 application guide (PDF, 5 MB)

 

 

 

Responsible Persons

If you have questions about the device please contact the responsible person. For training requests register here.

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

Weiterführende Informationen

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