Slidescanner - Zeiss Axio Scan.Z1 (Schlieren)
The Axio Scan.Z1 is an advanced slide scanner designed for the automated imaging of large volumes of slides, including both brightfield and multi-channel fluorescence specimens. Notably, the Axio Scan.Z1 is equipped with high numerical aperture dry objectives, ensuring exceptional resolution, and it possesses the capability to scan entire 3D volumes.
Location
University Zurich, Campus Schlieren , WAD H127 (325)
Wagistrasse 12, 8952 Schlieren
Training Request
Follow this link to apply for an introduction to the microscope.
Technical Specifications
Microscope
- Automated upright slide scanning microscope
- Slide holders for standard slides (26 x 76mm) or big slides (52 x 76mm) upon request
Light Sources
- Transmitted light LED
- HXP fluorescence lamp
Objectives
| Name | Magnification | NA | Immersion | WD |
| Fluar | 2.5x | 0.12 | air | |
| Fluar | 5x | 0.25 | air | 12.5 mm |
| Plan Apochromat | 10x | 0.45 | air | 2.1 mm |
| Plan Apochromat | 20x | 0.8 | air | 0.55 mm |
| Plan Apochromat | 40x | 0.95 | air | 0.25 mm |
Fluorescence Filters
| Name | Excitation | Beam splitter | Emission |
|---|---|---|---|
| Dapi | 385 | FT 420 | BP 450/40 |
| CFP | 436/20 | FT 455 | BP 480/40 |
| eGFP | 470 | FT 495 | BP 525/50 |
| YFP | 500/25 | FT515 | BP 535/30 |
| Cy3 | 555 | FT 570 | BP 605/70 |
| mRFP | 590 | FT 605 | BP 647/70 |
| Cy5 | 630 | FT 660 | BP 690/50 |
Camera Systems
- Hitachi HV-F202FCL camera 8bit (color camera), 1600 x 1200 pixels (2 MP), pixel size 4.4 µm
• Hamamatsu Orca Flash 4.0 monochrome camera 16bit (fluorescence camera), 2048 x 2048 pixels (4 MP), pixel size 6.5 µm
Accessories
- ZEN blue (installed on all VMs)
- ZEN Image Analysis module (installed on Special VM1)
- CZI to .TIFF converter (installed on all VMs)
- ZEN Advanced Processing and Analysis module and macro environment (Python) for the setup of feedback experiments
Remarks
Literature and Links
For more in-depth information about ZEN blue:
ZEN 2 slidescan module guide (PDF, 13 MB)
Useful tips and tricks to optimize your scan profiles:
Axio Scan.Z1 application guide (PDF, 5 MB)
Responsible Persons
If you have questions about the device please contact the responsible person. For training requests register here.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy