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Center for Microscopy and Image Analysis

Widefield - Nikon Ti2 (Balgrist Campus)

Nikon Eclipse Ti2

This inverted widefield system is suited to image live cells as well as fixed fluorescence and histologically stained samples. A temperature and CO2 controlled environment is possible for live cell experiments.

Location

University Zurich, Balgrist Campus, Lengghalde 5, 8008 Zurich, Room Microscopy W.112

Training Request

Follow this link  to apply for an introduction to the microscope.

Technical Specifications

Microscope

Nikon Eclipse Ti2, inverted

 

Light Sources

  • Lumencor sola light engine
Name Magnification NA Immersion          WD (mm)
Plan Fluor PhL DL   4x                            0.13 Air 16.5
Plan Fluor Ph1 DL   10x 0.3                Air 5.2
Plan Apo l Ph2 DM   20x 0.75 Air 1.0
S Plan Fluor ELWD DIC N1   40x 0.6 Air 3.6
Plan Fluor DIC H N2   40x 1.3 Oil 0.24
Plan Apo VC WI DIC N2   60x 1.2 Water 0.31

Ph = phase contrast, DL = Dark Low Contrast, DM = Dark Medium Contrast, DIC = Differential Interference Contrast

 

Fluorescence Filters

Name Excitation  Dichroic Emission
DAPI BP 377/50 409 BP 447/60
GFP BP 466/40 495 BP 525/50
YFP BP 500/24 520 BP 542/27
Cy3 BP 531/40 562 BP 593/40
Cy5 BP 628/40 660 BP 692/10

Camera Systems

  • cooled Nikon monochrome fluorescent DS-Qi2 CMOS camera

       4908 x 3264 pixel (16.25 MP)

       pixel size 7.3 um

       25 mm FOV

       Frame rate: 6 fps (4908×3264) up to 45 fps (1636×1088)

  • Nikon Colour DS-Ri2, CMOS

Pixel: 4908 x 3264 pixel (16.25MP)

Pixel size: 7.3 µm

16bit

Frame rate: 6 fps (4908×3264) up to 45 fps (1636×1088)

Accessories

  • IR Hardware Autofocus (Nikon Perfect Focus System - PFS)
  • Temperature and CO2/O2 control (Okolab bold line)
  • NIS-Elements with JOBS module (enabling e.g. automatic screening of 96 or 384 well plates)

 

Responsible Persons

If you have questions about the device please contact the responsible person.

 

Make sure to acknowledge the Center for Microscopy and Image Analysis in your publication to support us.

How to acknowledge contributions of the Center for Microscopy and Image Analysis

 

Weiterführende Informationen

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