Electrophoretic Tissue Clearing System - X-Clarity (Irchel)
The X-CLARITYTM Electrophoretic Tissue Clearing System is used for rendering tissues and organs optically transparent in minimal time.
In order to achieve transparency, samples fixed with formaldehyde are embedded in acrylamide gel to covalently link biomolecules into sturdy hydrogel network. Lipid bilayers, a major cause of tissue opacity, are broken up and removed through electrophoresis in the presence of ionic detergents.
The X-CLARITY system is designed to generate a dense uniform electric field. A timer, temperature sensor and a buffer circulation pump are added for the precise control of clearing conditions.
X-CLARITY tissue processing conserves fluorescence of genetically encoded proteins such as eGFP, tdTomato etc. and is compatible with further immunostaining.
Location
University Zurich, Irchel Campus, Room Y42-H-90
Training Request
Follow this link to apply for an introduction to the system.
Technical Specification
Body
The clearing system consists of the power and temperature controller, chamber with electrodes, pump and a buffer reservoir.
Specifications
|
Current range |
0.2 – 1.5A (adjustable) |
|
Voltage range |
0 – 60V (not adjustable, device is designed to generate constant current) |
|
Temperature control |
Below 50°C |
|
Timer |
0 – 600 min |
|
Reservoir volume Pump speed Internal chamber volume |
2L 0.1- 100 rpm 57 x 30 x 93 mm |
Accessories
Tissue containers for:
- whole adult mouse brain;
- tissue slices up to 4mm;
- whole rat brain;
- customized holder for various mouse organs.
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy