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Center for Microscopy and Image Analysis

CLSM - Evident Fluoview 4000 (Irchel)

The Evident Fluoview 4000 confocal laser scanning microscope is a high-end confocal imaging systems equipped with 

  • SilVIR Next-Generation Detector Technology (Silicon photomultiplier (SiPM).
  • Extented near infrared (NIR) lasers will allow to expand your spectrar range from 405nm to 785 nm, making it possible to multiplex up to six channels simultaneously.
  • Resonant scanner allowing high speed imaging. 
  • Stage top incubation system allows control of temperature and CO2 / Olevels enabling live cell experiments.

Location

University Zurich, Irchel Campus, Room Y42-H-93.

Training Request

Follow this link to apply for an introduction to the microscope.

Technical Specifications

Microscope

  • inverted microscope (Olympus Inverted IX83)
  • motorized xyz stage (IX3-SSU)
  • Olympus Z-drift compensator (IX3-ZDC2)

Light Sources and Lasers

  • Epifluorescence Lightsource
    • solid state LED and LDP - U-LGPS
    • 360 to 665 nm
  • Laser lines for confocal imaging:
    • 405 nm, 445 nm, 488 nm, 561 nm, 594 nm, 640 nm
    • NIR: 685 nm, 730 nm, 785 nm

Objectives

Name Magnification NA Immersion WD (mm)
PlanApo N 2x 0.08 Air 6.2
UCPLFLN20X 20x 0.7 Air 0.80 - 1.80
UPlanXApo 40x 0.95 Air 0.180

Silicon oil Immersion

        
UPLSAPO UPlan S Apo 30x 1.05 Silicon oil 0.8

UPLSAPO UPlan S Apo

60x 1.3

Silicon oil

0.3

Fluorescence Filters for Epifluorescence

For further details please contact the responsible persons.

 

Detectors

Accessories

  • Inserts for ibidi slides, well plates and 35mm dishes
  • Incubation system for live cell imaging (cellVIVO)
  • Allows for a variety of experimental protocols with 0 - 20 % CO2.
  • Reliable control of temperature and CO2 with up to 0.05 °C accuracy

Literature and Links

 

Responsible Persons

If you have questions about the device please contact the responsible person.

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy

Weiterführende Informationen