CLSM - Leica SP8 inverse FALCON (Irchel)
The Leica SP8 is an automated inverted confocal laser scanning microscope allowing simultaneous acquisition of 5 fluorescent channels and 1 transmitted light channel (BF). The system is equipped with a very flexible white light laser source and an additional 440 nm pulsed laser. Further, the SP8 FALCON (Fast Lifetime CONtrast) allows for Fluorescence Lifetime Imaging (FLIM) faster than conventional systems. A resonant scanner, climate chamber, including temperature and CO2/O2 controller (for hypoxia), favor confocal live cell studies.
Location
University Zurich, Irchel Campus, Room Y42-H-81.
Training Request
Follow this link to apply for an introduction to the microscope.
Technical Specifications
Microscope
- inverted confocal microscope (Leica DMi8 CS with AFC, Model SP8)
- 5 freely selectable emission detection windows
- motorized x/y stage
- 3D capability
- time lapse capability
Light Sources and Lasers
- Halogen lamp for transmitted light
- External fluorescence lamp LQ-HXP 120
- Diode Lasers: 405 nm (50mW)
- 440 nm laser: pulsed with a flexible repetition rate up to 40 MHz
- White light laser (WLL): continuous laser output in the range of 470-670nm; AOTF allows to choose up to 8 different wavelengths simultaneously; pulsed laser system with a flexible repetition rate up to 80 MHz (pulse picker)
Scanners (tandem scanner system)
- confocal point-scanning - optical sectioning
- zoom function
- scan field rotation
- Regular scanner (field-of-view scanner): 1 Hz to 1800 Hz
- Resonant scanner: 8 kHz
Detectors
- 5 confocal fluorescence channels: two Photomultipliertubes (PMTs) and three super-sensitive FLIM capable photon-counting hybrid detectors (SMD HyDs, suitable for time gated detection)
- one transmission light-channel (PMT-Trans)
Objectives
| Name | Magnification | NA | Immersion | WD (mm) |
|---|---|---|---|---|
| HC PL FLUOTAR | 10x | 0.3 | Air | 11.0 |
| HC PL APO corr CS2 | 20x | 0.75 | IMM | 0.68 |
| HC PL APO corr CS2 | 63x | 1.3 | Glycerol | 0.3 |
| HC PL APO CS2 | 63x | 1.4 | Oil | 0.1 |
| HC PL APO CS2 | 40x | 1.1 | Water | 0.65 |
| HC PL APO CS2 | 20x | 0.75 | Air | 0.62 |
CS2 objectives: improved color correction, perfect VIS-405; IMM = multi immersion (either water, glycerol or oil)
Fluorescence Filters
| Name | Excitation Range | Excitation Filter | Dichroic | Emission Filter |
|---|---|---|---|---|
| DAPI | UV | BP 350/50 | 400 | BP 460/50 |
| GFP | blue | BP 470/40 | 495 | BP 525/50 |
| RHOD | green | BP 546/10 | 560 | BP 585/40 |
Accessories
- Controlled environmental conditions (CO2 /O2, for studies under hypoxia, temperature, humidity)
- Ludin chambers for 12 and 18 mm cover glasses (reservation needed)
- Matrix screening software (reservation needed)
- Holders for slides and well plates
Literature and Links
Responsible Persons
If you have questions about the device please contact the responsible person.
Make sure to acknowledge the Center for Microscopy in your publication to support us.
How to acknowledge contributions of the Center for Microscopy