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Center for Microscopy and Image Analysis

CLSM - Leica SP8 inverse (Gloriastrasse)

SP8_Gloriastrasse

The Leica SP8 is an automated inverted confocal laser scanning microscope allowing simultaneous acquisition of 5 fluorescent channels and 1 transmitted light channel (BF or DIC). Further, the system is outfitted with a very flexible white light laser source. A climate chamber and resonant scanner favor confocal live cell studies.

Location

University Zurich, Gloriastrasse 30, Room GLM F01.

Training Request

Follow this link  to apply for an introduction to the microscope.

Technical Specifications

Microscope

  • inverted confocal microscope (Leica DMi8 CS with AFC, Model SP8)
  • 5 freely selectable emission detection windows
  • motorized x/y stage
  • 3D capability
  • time lapse capability

Light Sources and Lasers

  • Halogen lamp for transmitted light
  • External fluorescence lamp  LQ-HXP 120
  • Diode Lasers: 405 nm (50mW), 442nm
  • Argon Laser (65mW): 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
  • White light laser (WLL): continuous laser output in the range of 470-670nm; AOTF allows to choose up to 8 different wavelengths simultaneously; pulsed laser system with a repetition rate of 80 MHz

Scanners (tandem scanner system)

  • confocal point-scanning - optical sectioning
  • zoom function
  • scan field rotation
  • Regular scanner (field-of-view scanner): 1 Hz to 1800 Hz
  • Resonant scanner: 8 kHz

Detectors

  • 5 confocal fluorescence channels: one Photomultipliertubes (PMTs) and four super-sensitive Hybrid detectors (HyD)
  • one transmission light-channel (PMT-Trans) 

Camera System

  • Hamamatsu Orca Flash 4.0 V3 high sensitivity, digital monochrome scientific cooled sCMOS camera
  • QE > 82% @ 560 nm
  • 2048 x 2048 pixel (6.5 um x 6.5 um)
  • 16 bit dynamics

Objectives

Name Magnification NA Immersion WD (mm)
HC PL FLUOTAR 10x 0.3 Air 11.0
HC PL APO CS2 20x 0.75 IMM 0.68
HC PL APO 40x 0.95 Air 0.17
HC PL APO CS2 63x 1.4 oil 0.1
HC PL APO CS2motCorr 63x 1.2 water 0.3

CS2 objectives: improved color correction, perfect VIS-405; IMM = multi immersion (either water, glycerol or oil)

Fluorescence Filters

Filter for Epi-Fluorescence using the Filter turret

Name Excitation Range Excitation Filter Dichroic Emission Filter
DAPI UV BP 350/50 400 BP 460/50
GFP blue BP 470/40 495 BP 525/50
RHOD green BP 546/10 560 BP 585/40
QUAD-S UV/blue/green/red LP (see fast filter wheels) 425; 505; 575; 660 LP (see fast filter wheels)

Filter for Epi-Fluorescence using the QUAD-S in combination with the fast filter wheels

Filter Name Excitation/Emission Range Filters/Bands
DFTC-EX UV/blue/green/red BP 350/50, BP 490/20, BP 555/25, BP 645/30, EMPTY
DFTC-EM UV/blue/green/red BP 455/50, BP 525/36, BP 605/52, BP 705/72, EMPTY

 

Accessories

  • controlled environmental conditions (CO2, temperature, humidity)
  • Matrix screening software (reservation needed)

Literature and Links

Laser Scanning confocal Microscopy

ibiology - Optical Sectioning and Confocal Microscopy  

Leica Science Lab

 

Responsible Persons

If you have questions about the device please contact the responsible person.

 

Make sure to acknowledge the Center for Microscopy in your publication to support us.

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Weiterführende Informationen