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Center for Microscopy and Image Analysis

Lightsheet Zeiss Lattice Lightsheet (Irchel)

ZEISS Lattice Lightsheet

We are excited to offer the ZEISS Lattice Lightsheet 7 as a demo system, generously provided by ZEISS, free of charge for all trained users from mid-October 2024 to mid-January 2025.

The ZEISS Lattice Lightsheet 7 offers a fast and gentle light sheet fluorescence microscopy solution with an inverted configuration, designed for live and fixed sample imaging. The system is compatible with standard sample carriers featuring coverslip bottoms (#1.5), including 35 mm glass-bottom dishes, Labtek chambers, and IBIDI slides. Equipped with three laser lines (488 nm, 561 nm, and 640 nm), two Hamamatsu Fusion sCMOS cameras, and a 48x/1.0 NA detection objective, it enables subcellular resolution imaging with minimal photo damage. Fully incubated for live cell experiments, the system integrates acquisition and data processing through ZEN Blue, ensuring smooth workflows for extended imaging sessions.

Applications:

  • Live cell imaging over extended periods
  • High-volume imaging at fast speeds
  • Single plane acquisition for high frame rates

Responsible Person

responsible person

Location

University Zurich, Irchel Campus, Room Y42 H36. 

Training Request

 Follow this link to apply for an introduction to the microscope

Light-sheet microscopy

Lightsheet

 


Light sheet microscopy, also known as Gaussian light sheet microscopy, is widely recognized for its ability to provide gentle imaging conditions at high speeds. Its innovative approach separates excitation from detection, enabling illumination of only the portion of the specimen within the focal plane of the detection objective. By moving the light sheet relative to the sample and capturing an image at each focal plane, it allows for the acquisition of volumetric data while minimizing exposure to out-of-focus areas of the sample.

Lattice-Light-sheet

lattice

 
Lattice light sheet microscopy merges the benefits of traditional light sheet microscopy with near-isotropic resolution comparable to confocal imaging. By employing advanced beam-shaping technology, it generates lattice-shaped light sheets that are much thinner than conventional Gaussian light sheets, offering enhanced resolution without sacrificing imaging speed. This lattice pattern is formed using a Spatial Light Modulator (SLM) and is projected onto the sample after being refined by scanners, which dither the lattice to create a uniform, smooth light sheet for imaging.

 

Technical Specifications

Microscope body

  • inverted microscope 

  • Five-axis multi-coordinate stage with Piezo motors

Light Sources and Lasers
  • Solid state diode lasers:
    • 488 nm (10mW)
    • 561 nm (10 mW)
    • 642 nm (5 mW)

Camera System

  • 2x sCMOS Hamamatsu Orca Fusion C14440-20UP

  • 2304 × 2304 (5.3 Megapixels)

  • 100 frames/s (at 2304 × 2048 ROI)

  • Pixel size : 6.5 μm × 6.5 μm
  • Peak QE : 80 %

Environmental control

  • Heating System (ambient to 42 °C)

  • Gas Mixer System (CO₂: 0 % to 15 % ±0.35 %, O₂: 1 % to 21 % ±0.20 %, Humidity: 20 % – 99 % ±2.50 %)

Accessories
  • Inserts for petri dishes (35 mm), for standard object slides, µ-Slides by Ibidi, Nunc Lab-Tek Slides and Chambers by Thermo Scientific

Available Optics
Name Magnification NA Immersion WD (mm)

ZEISS 

48x

1.0

Water

 

 

Links & Literature

ZEISS Lattice Light-sheet

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