Multiphoton - Leica SP8 MP DIVE FALCON (Irchel)

The upright Leica multiphoton and confocal fluorescence microscope is equipped with a pre-compensated infrared laser (Insight DS+ Dual from Spectra Physics) for non linear fluorescence excitation and second harmonics generation as well as with a white light laser for single photon excitation.

The 4 channel tuneable non-descanned DIVE detection unit allows for unprecedented spectral flexibility in multiphoton experiments. 

Six FLIM enabled HyD detectors together with the fast FALCON FLIM implementation allow for highly efficient multidimensional FLIM imaging in confocal as well as multiphoton operation.

University Zurich, Irchel Campus, Room Y42-H-77. 

Follow this link to apply for an introduction to the microscope.

Light Sources and Lasers

• Insigth DS+ Dual (680-1300 nm & 1041nm) ultrafast NIR laser for multiphoton excitation
• White light laser (WLL): continuous laser output in the range of 470-670nm; AOTF allows to choose up to 8 different wavelengths simultaneously; pulsed laser system with a flexible repetition rate up to 80 MHz (Pulse picker)
• Diode laser 405 nm (50mW)
• Argon Laser (65mW): 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
• Halogen lamp for transmitted light
• External fluorescence lamp  LQ-HXP 120

Objectives

Widefield Fluorescence Filters

• DAPI
• GFP (Installed)
• CFP
• DsRed
• Polarisator for phase contrast

FLIM Hardware

• Fully integrated Leica fast lifetime contrast (FALCON) FLIM implementation
• 4 non-descanned FLIM capable HyD detectors
• 2 descanned FLIM capable HyD detectors
• Single photon excitation with supercontinuum laser (Variable repetition rate)
• Multiphoton excitation (80 MHz)
• TCSPC modalities: FLIM / FLIM FRET / Phasor Analyis

Camera Systems

Leica DFC7000 T Color CCD Camera

• digital color, high-sensitivity, cooled camera for fluorescence and brightfield microscopy
• 1920x1440 pixel image size
• RGB Bayer mask color filter
• 4 microseconds - 200 seconds exposure
• 8 or 12 bit bit depth
• 40 fps @ full frame
• color or monochrome operation

Accessories

• Ludin light insulation box
• Flexible Scientifica Motorised Movable Base Plate (MMBP) with accessories
• CUDA accelerated inline Lightning deconvolution
• Optional Leica SuperZ galvanometric z-stage for fast and precise z-positioning
• Trigger box for triggering image acquisition or external hardware components

Literature and Links

Olympus multiphoton primer

https://dx.doi.org/10.1038/nmeth818

http://www.opticsinfobase.org/josaa/abstract.cfm?uri=josaa-23-12-3139

Further information (internal UZH use only)

Follow this link for further background information, documents and links.

Responsible Persons

If you have questions about the device please contact the responsible person.

Make sure to acknowledge the Center for Microscopy in your publication to support us.

How to acknowledge contributions of the Center for Microscopy