Multiphoton - Leica TCS SP8 MP (Botinst)

The inverted Leica multiphoton and confocal fluorescence microscope is equipped with a pre-compensated infrared laser (Insight DS+ Dual from Spectra Physics) as well as with a whitelight laser for visible excitation. Besides multiphoton excitation as well as one photon excitation, non-linear experiments such as second harmonic generation can be performed with the system.

BOT Room M2-21, Zollikerstrasse 107, 8008 Zürich.

Follow this link to apply for an introduction to the microscope.

Light Sources and Lasers

• Insigth DS+ Dual (680-1300 nm & 1041nm) ultrafast NIR laser for multiphoton excitation
• White light laser (WLL): continuous laser output in the range of 470-670nm; AOTF allows to choose up to 8 different wavelengths simultaneously; pulsed laser system with a repetition rate of 80 MHz
• Diode laser 405 nm (50mW)
• Argon Laser (65mW): 458 nm, 476 nm, 488 nm, 496 nm, 514 nm
• Halogen lamp for transmitted light
• External fluorescence lamp  LQ-HXP 120

Objectives

Fluorescence Filters

Non-Descanned Detection:

• HyD-RLD 2ch DAPI/FITC filter cube: 460/50 & 525/50 with 495 SP
• HyD-RLD 2ch CFP/YFP filter cube: 483/32 & 535/30 with 505 SP
• HyD-RLD 2ch TRITC/A633 filter cube: 585/40 & 650/50 with 620 SP
• HyD-RLD 2ch A488/A647 filter cube: 525/50 & 675/55 with 620 SP

• Beamsplitter Quad LP 505
• Beamsplitter Quad SP 500
• Beamsplitter Quad LP 560
• Beamsplitter Quad SP 565

Widefield Fluorescence Filter Cubes

• DAPI
• GFP
• CFP
• DsRed

Camera Systems

Leica DFC7000 T Color CCD Camera

• digital color, high-sensitivity, cooled camera for fluorescence and brightfield microscopy
• 1920x1440 pixel image size
• RGB Bayer mask color filter
• 4 microseconds - 200 seconds exposure
• 8 or 12 bit bit depth
• 40 fps @ full frame
• color or monochrome operation

Accessories

• controlled environmental conditions (temperature, humidity)

Literature and Links

http://www.olympusmicro.com/primer/techniques/fluorescence/multiphoton/multiphotonintro.html

https://dx.doi.org/10.1038/nmeth818

http://www.opticsinfobase.org/josaa/abstract.cfm?uri=josaa-23-12-3139

Further information (internal UZH use only)

Follow this link for further background information, documents and links.

Responsible Persons

If you have questions about the device please contact the responsible person.

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