Navigation auf uzh.ch

Suche

Center for Microscopy and Image Analysis

Fluigent Aria (Irchel)

Fluigent Aria (Irchel)

The Fluigent Aria system is an advanced microfluidic flow controller designed to automate and optimize fluid delivery for various microscopy applications, including multiplexed imaging, automated immunolabeling and DNA-PAINT workflows. Its precise pressure-based control allows reliable reagent delivery, minimizing sample loss while ensuring reproducibility. The Aria is particularly well-suited for applications requiring multi-channel fluid handling, such as spatial biology, live-cell imaging, and tissue staining.
 

  • Automated Fluid Delivery: Controls up to 10 fluidic channels for sequential or parallel reagent delivery.
  • Precision Flow Control: Pressure-based regulation ensures stable flow rates with ±5% accuracy and ±0.5% repeatability.
  • Compatible with Imaging Systems: Seamless integration with TTL-enabled widefield and confocal microscopes.
  • Minimal Reagent Waste: Low dead volume and customizable flow paths reduce sample and reagent consumption.
  • Reproducible Results: Automated protocols eliminate manual pipetting variability.
  • Flexible Integration: Supports microfluidic chips, perfusion chambers, and tissue slides.

Responsible Person

responsible person

Location

University Zurich, Irchel Campus, Room Y38-M-77.

Training Request

 Follow this link to apply for an introduction to the microscope

 

Applications
  • multiplexed imaging
  • automated immunolabeling
  • DNA-PAINT

 

Technical Specifications

Channel Configuration

 Supports up to 10 independent fluidic channels with flexible control.

M-Switch Valve

 Multi-channel output for applications requiring multiple flow paths, such as perfusing multiple chips simultaneously.

Flow Rate Range

 40 µL/min to 1 mL/min (Accuracy: ±5% above 40 µL/min. and Repeatability: ±0.5%.)

Pressure Control

 Regulated up to 2.4 bar (34.8 psi)

Stop Flow Functionality

 Integrated on-off valve to halt flow instantly during imaging or incubation steps.

Wetted Surfaces

 Polypropylene, FEP, Glass, PEEK, and other materials ensure compatibility with most aqueous solutions and buffers.

Reservoirs
  • Standard: 8 × 15 mL (ideally FALCON)
  • Convertible: Adapters available for 2 mL reservoirs.
  • Flushing Reservoir: 2 × 100 mL for waste and cleaning solutions

Reservoir compatibility
  • 1.5 mL and 2 mL Eppendorf
  • 15 mL and 50 mL Falcon

Tubing

  • Material: FEP (1/16" OD, 250 µm ID).

  • Chemical Compatibility: All reagents must be compatible with wetted surfaces (Polypropylene, FEP, Glass, PEEK). Avoid corrosive or caustic substances.

User Guide

 will come soon!

Links & Literature

Fluigent Aria
Aria User manual Download Aria User Manual

Make sure to acknowledge the Center for Microscopy and Image Analysis in your publication to support us.

How to acknowledge contributions of the Center for Microscopy and Image Analysis