Navigation auf uzh.ch
Navigation auf uzh.ch
At the Center for Microscopy and Image analysis we provide user support for all phases of your spatial biology-multiplexing project : from experimental design, sample preparation to imaging and processing of the resulting data.
We also closely collaborate with the other technology platforms at the University of Zurich and will support you in finding the best technology that fits your spatial-omic research.
Currently we offer different instruments that are suitable for Spatial biology / Multiplexing workflows:
New in 2025: The Lunaphore COMET is now located at WAD-K-134, the 5th floor of UZH Schlieren
A custom image processing pipeline for stitching and registering raw images in 3D can be found at:https://github.com/ZMB-UZH/zmb-ibex-jove
We at the Center for Microscopy and Image Analysis are thrilled to announce that the Lunaphore COMET system is now at Schlieren and will be operational starting in February of 2025.
To mark this exciting milestone, we invite you to join us for two in-person seminars:
Introduction to Multiplex Immunofluorescence (seqIF) on the COMET
📅 Date: Tuesday, February 4, 2025
🕚 Time: 11:00 AM
📍 Location: Seminar room WAD-J-121/123 4th floor, Schlieren
Exploring Spatial Multiomics with RNAscope HiPlex Pro on the COMET
📅 Date: Thursday, February 6, 2025
🕥 Time: 10:30 AM
📍 Location: Seminar room WAD-L-121/123 6th floor, Schlieren
In addition, the ZMB together with Lunaphore’s field application specialist, Clément Chevalier, will host streamed demonstrations of system operation workflows and data processing from Tuesday afternoon through Friday morning of the same week. You can join these demonstrations using the following [Teams link].
For more details about the seminars, the streamed sessions, and the week’s training schedule, please read below and check back here for updates.
We look forward to arranging individual trainings for you on the Lunaphore COMET this winter, please reach out to our Multiplexing Specialist, Olivia Hanley at olivia.hanley@zmb.uzh.ch to schedule a training on the system so we can accelerate your spatial biology projects in 2025.
Please save the dates and feel free to share this information with any potential users of the Lunaphore COMET system.
For those interested in streaming the COMET trainings throughout the week of February 3rd the schedule is as follows:
The afternoon of Tuesday February 4th. 13:15-17:00- Setup the Marker Characterization reagents and protocol on the COMET software. Buffer and Reagent Prep, and System Operation. End with Q&A and wrap-up.
The morning of Wednesday February 5th. 9:30-12:15- Prep for 10-plex and own sample seqIF, and data visualization from previous days results with the viewer software.
The afternoon of Wednesday February 5th. 13:30- 16:45 Second 10-plex seq IF run, buffer and reagent preparation, operate and start an overnight run. End with Q&A and wrap-up.
The morning of Thursday February 6th. 9:30-10:30- Review 10-plex seqIF data visualization from previous days results with the viewer software. 10:30 Seminar L 121/122 6th floor.
The afternoon of Thursday February 6th. 13:30- 16:45 Multiomics protocol design and prepare sample reagents adn protocol on the COMET software, system buffer and reagent prep, start an overnight run. End with Q&A and wrap-up.
The morning of Friday February 7th. 9:30-12:30- Visualize and discuss the Multiomics data followed by an introduction to Horizon analysis software. Wrap-up.
This is the link to the streamed training over Teams.
What multiplexing method are you interested in? At the ZMB we have worked with users to run 4i, HiFi, CODEX, and IBEX protocols on our existing infrastructure. The image above is from 5 rounds of 4i imaged on the Zeiss Airyscan confocal. Let's discuss how we can help you with your spatial biology project!
Interested in automating your staining and imaging using the Fluigent Aria microfluidic pump with the existing ZMB infrastructure, let's discuss how we can integrate this tool into your multiplexing workflow.
