Navigation auf uzh.ch
Navigation auf uzh.ch
| Sequential Immunofluorescence (seqIF) | seqIF is a fully automated imaging technique that enables the detection of multiple protein biomarkers within a single tissue sample. This method involves iterative cycles of staining with non-conjugated primary antibodies, imaging, and gentle elution of antibodies, preserving tissue integrity throughout the process. Each cycle allows for the detection of different antigens, facilitating high-plex spatial proteomics analysis. By maintaining spatial context and enabling extensive multiplexing, seqIF™ serves as a valuable tool for detailed cellular and tissue analysis. |
| iterative Bleaching Extends Multiplexity (IBEX) | IBEX is a high-content imaging technique that enables visualization of over 65 biomarkers within a single tissue sample. This method involves repeated cycles of antibody labeling, imaging, and chemical bleaching. After each imaging round, fluorophores are chemically bleached, effectively removing their signals while preserving tissue integrity. This process allows for subsequent rounds of staining without spectral overlap. IBEX is compatible with a wide range of commercially available antibodies and fluorophores, making it versatile for various research applications. By maintaining spatial context and enabling extensive multiplexing, IBEX serves as a valuable tool for detailed cellular and tissue analysis. |
| Co-Detection by Indexing (CODEX) | CODEX is a high-parameter imaging technology that enables simultaneous visualization of up to 60 biomarkers within a single tissue sample. This method utilizes antibodies conjugated to unique DNA oligonucleotides, which bind to their target antigens in the tissue. Fluorescently labeled complementary DNA probes are then introduced in cycles to hybridize with these oligonucleotides, allowing for the sequential imaging of multiple markers. After each imaging cycle, the fluorescent probes are removed, and new probes are applied, facilitating extensive multiplexing without compromising tissue integrity. CODEX is compatible with both formalin-fixed, paraffin-embedded (FFPE) and fresh-frozen tissues, making it a versatile tool for detailed spatial analysis of complex tissues. |
| Iterative Indirect Immunofluorescence Imaging (4i) | Iterative Indirect Immunofluorescence Imaging (4i) is a technique that enables the visualization of multiple proteins within a single tissue sample. It involves cycles of indirect immunofluorescence staining, imaging, and antibody removal. After imaging, antibodies are gently eluted using mild conditions that preserve sample integrity. This process allows for the sequential detection of numerous proteins without degrading the tissue. 4i utilizes standard, commercially available primary and secondary antibodies, eliminating the need for custom labeling. By maintaining spatial context and enabling extensive multiplexing, 4i serves as a valuable tool for detailed cellular and tissue analysis. |
Make sure to acknowledge the Center for Microscopy and Image Analysis in your publication to support us.
How to acknowledge contributions of the Center for Microscopy and Image Analysis
