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Center for Microscopy and Image Analysis

Lunaphore COMET (Schlieren)

Lunaphore COMET

The Lunaphore COMET system is a fully automated, high-throughput multiplex immunofluorescence platform designed for spatial biology and tissue profiling. By integrating microfluidics, staining, and imaging in one streamlined workflow, COMET enables the analysis of multiple biomarkers (40+ plex) on a single tissue section without compromising sample integrity.

Responsible Person

responsible person

Location

University Zurich, Schlieren Campus, Room WAD-12-K-105.

Training Request

 Follow this link to apply for an introduction to the microscope

Applications

 Multiplex sequential immunofluorescence (seqIF) and RNAscope HiPlex
Plex-level
  • Virtually unlimited plex level capability of technology
  • Proteomics application: performs 40-plex protocols per automated run
  • Multiomics application: performs 12 RNA plus up to 24 protein targets, or 4 RNA plus up to 28 protein targets
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Technical Specifications

Slide capacity

4 slides

Slide compatibility

 Standard histology slides - 75 mm x 25 mm (3 in x 1 in), 1 mm thick, positively charged

Sample thickness

 3-10 μm

Automation level

 Fully automated: staining, target probe hybridization, image acquisition and image preprocessing

Maximum imaging area 

 12.5 mm x 12.5 mm

Maximum staining area

 21 mm x 21 mm

Throughput

 Measured for a 9 mm x 9 mm imaging area: 20 slides/week for 20-plex protein panel ; 12 slides/week for 40-plex protein panel

Reagents

 Open choice of primary antibodies (non-conjugated):
  • Offered by Lunaphore: SPYRE™ Antibody Panel kits & Tumor Markers
  • Other standard primary antibodies of choice
Other reagents and buffers:
  • Offered by Lunaphore: Secondary Antibodies, Buffers, DAPI
  • Alexa Fluor™ Plus matching FITC, TRITC, Cy5 and Cy7 channel characteristics are highly recommended as detection systems
  • Offered by ACD: RNAscope™ HiPlex Pro detection kit, RNAscope™HiPlex probes

Software
  • COMET™ Control Software – for instrument operation
  • Panel Builder – online application for automated generation of ready-to-use protocols
  • HORIZON™ Viewer– for image visualization and quality control
  • HORIZON™ Software – for image analysis (optional)
  • Oncotopix® Discovery (Visiopharm)
  • HALO® & HALO, AI™ (Indica Labs)
  • Nucleai AI-powered solutions
  • QuPath

Available Optics

 20X / 0.7 NA

Image resolution

 0.28 µm/pixel

Available Channels

 DAPI, FITC, TRITC, Cy5, Cy7

Output file format

 OME-TIFF
   

User Guide

 will come soon!

Links & Literature

Lunaphore COMET

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