For high resolution tile imaging of large samples the following microscopy systems are available:
mesoSPIM V6 "Revolver"
CLSM - Leica SP8 upright
Multiphoton - Leica TCS SP8 Upright MP
The advantages of mesoSPIM system:
- suitable for whole-mount cm-size samples, such as mouse brains
- fast imaging, e.g. from 0.5 to 3 hrs per mouse brain, depending on magnification
- compatibility with multiple clearing techniques and refractive indices, from water (n1.33) to BABB (n1.56)
The advantages of Leica SP8 systems:
- fully automated stage
- upright systems allow imaging without using a coverslip
- multiphoton system is equipped with high-performance objectives (including 2 dedicated high-NA, long working distance multiphoton objectives)
